Luk KF, Ko KM, Ng KM.
 Bioengineering Graduate Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
 Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
 Department of Chemical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
Schisandrin B (Sch B) is the most abundant and biologically active dibenzocyclooctadiene lignan present in Fructus Schisandrae (FS). It is a highly desirable ingredient for a dietary supplement. While Sch B has been isolated from FS in the laboratory, the experimental procedure was usually lengthy and the yield was relatively low. The objective of this study is to develop a process that is amenable to large-scale recovery of Sch B from FS. The process starts with solid–liquid extraction of FS by petroleum ether to yield a Sch B-containing extract, which is then purified by removing the relatively nonpolar substances, mostly fatty oils, by an adsorption–desorption step. This is followed by a chromatographic process to isolate the Sch B-enriched fraction that is then used to yield Sch B by crystallization.